Coding

Part:BBa_K2680538:Design

Designed by: Julia Urban   Group: iGEM18_William_and_Mary   (2018-10-15)


LacI-ssra


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.

Source

"Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). [...] Incompatible with systems containing LacI, lactose, or IPTG."1

References

1Kenney, G., Shen, D., Varshney, N., & Sutton, S. (2003, January 31). Part:BBa_C0012. Retrieved October 15, 2018, from https://parts.igem.org/Part:BBa_C0012 Sequence and Features